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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 충남대학교 농업과학연구소 Korean Journal of Agricultural Science Korean Journal of Agricultural Science Vol.45 No.4
- 발행연도
- 2018.12
- 수록면
- 715 - 723 (9page)
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초록· 키워드
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The present study was done to investigate the effect of co-culturing muscle satellite cells (MSCs) and intramuscular preadipocytes (IPs) on the differentiation of adipocytes and muscle cells isolated from Korean native cattle. MSCs and IPs were single-cultured in 10% fetal bovine serum/Dulbecco"s modified Eagles medium (FBS/DMEM) for 48 h followed by culturing in 5% FBS/DMEM as the growth media. Then, the growth media was replaced by differentiation media composed of 2% FBS/DMEM without any additives for the single- or co-culture of muscle cells and intramuscular adipocytes to induce the differentiation of both cell types. Cell differentiation was measured by morphological investigation and cytosolic enzyme analysis of glycerol-3-phosphate dehydrogenase (GPDH) for the adipocytes and creatine kinase (CK) for the muscle cells. In the morphological test, the presence of muscle cells did not stimulate adipocyte differentiation showing more differentiation of the adipocytes in the single-culture compared to the co-culture condition. However, the differentiation of muscle cells was promoted by adipocytes in the co-culture. The results of the enzymatic analysis were highly associated with the morphological results with a statistically higher GPDH activity (p < 0.05) appearing in the single-culture than in the co-culture, whereas the opposite was true for the CK activity of the muscle cells (p < 0.05). By manipulating in vivo the milieu using a co-culture, we could detect the difference in the rate of cell differentiation and suggest that a co-culture system is a more reliable and precise technique compared to a single-culture. Further studies on various co-culture trials including supplementation of differentiating substances, gene expression analysis, etc. should be done to obtain practical and fundamental data.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Conclusion
References
참고문헌 (29)
참고문헌 신청
[학술지(정기간행물)] - Ailhaud G / 1992 / Cellular and molecular aspects of adipose tissue development / Annual Review of Nutrition 12 : 207 ~ 233
[학술지(정기간행물)] - Brindle PK / 1992 / The CREB family of transcription activators / Current Opinion in Genetics and Development 2 : 199 ~ 204
[학술지(정기간행물)] - 최창원 / 2011 / Sera Taken from Aged Korean Native Steers Increase Adipocyte Differentiation / 농업생명과학연구 45 (2) : 85 ~ 92
[학술대회논문] - Choi CW / 2007 / Screening of media conditions to establish optimum conditions for 3T3-L1 cells co-cultured with L6 cells / Proceedings of Animal Science and Technology, Chungang University
[학술지(정기간행물)] - 최창원 / 2012 / 지방과 근육 세포주의 단독 및 공동배양을 통한 세포형태학 및 세포물질 비교 연구 / 한국축산학회지 54 (2) : 103 ~ 109
[학술지(정기간행물)] - Brindle PK / 1992 / The CREB family of transcription activators / Current Opinion in Genetics and Development 2 : 199 ~ 204
[학술지(정기간행물)] - 최창원 / 2011 / Sera Taken from Aged Korean Native Steers Increase Adipocyte Differentiation / 농업생명과학연구 45 (2) : 85 ~ 92
[학술대회논문] - Choi CW / 2007 / Screening of media conditions to establish optimum conditions for 3T3-L1 cells co-cultured with L6 cells / Proceedings of Animal Science and Technology, Chungang University
[학술지(정기간행물)] - 최창원 / 2012 / 지방과 근육 세포주의 단독 및 공동배양을 통한 세포형태학 및 세포물질 비교 연구 / 한국축산학회지 54 (2) : 103 ~ 109
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UCI(KEPA) : I410-ECN-0101-2019-520-000313640