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- 2016.1
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This study was conducted to identify the general conditions for the isolation and in vitro culture of ovary-derived cells in rockfish (Sebastes schlegeli). The effects of three different enzymes on cell retrieval from ovarian tissues were evaluated first, and then the ovary-dissociated cells were cultured under various culture conditions, with varying basal media and culture temperatures, addition of growth factors, and/or culture types. We found that collagenase type I treatment was effective for cell isolation from ovarian tissues. From a total of 42 trials to evaluate the effects of basal media and culture temperatures on cell culture of ovary-dissociated cells, we observed that Leibovitz’s L15 medium was more supportive than Dulbecco’s modified Eagle’s medium for culture, and the cells could grow at all three temperatures tested, 15, 20, and 25 °C, at least up to passage 2. However, growth factor addition did not improve cell growth. Introduction of suspension culture after monolayer culture expanded the culture period significantly more than did monolayer culture alone. Our results may provide a basis for developing an in vitro system for S. schlegeli germline cell culture, which will ultimately lead to improvement of the species.
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참고문헌 (31)
참고문헌 신청
Abdul Majeed S / 2013 / Establishment and characterization of permanent cell line from gill tissue of Labeo rohita(Hamilton)and its application in gene expression and toxicology / Cell Biol Toxicol 29:59~73
Ando M / 1999 / Non-steroidal anti-inflammatory drugs(NSAIDs)block the late, prostanoid-dependent/ceramide-independent component of ovarian IL-1 action : implications for the ovulatory process / Mol Cell Endocrinol 157:21~30
Bols NC / 1994 / Development of a cell line from primary cultures of rainbow trout, Oncorhynchus mykiss(Walbaum), gills / J Fish Dis 17:601~611
Can A / 2007 / Concise review : human umbilical cord stroma with regard to the source of fetus-derived stem cells / Stem Cells 25:2886~2895
Freshney RI. / 2010 / Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications / Wiley-Blackwell
Ando M / 1999 / Non-steroidal anti-inflammatory drugs(NSAIDs)block the late, prostanoid-dependent/ceramide-independent component of ovarian IL-1 action : implications for the ovulatory process / Mol Cell Endocrinol 157:21~30
Bols NC / 1994 / Development of a cell line from primary cultures of rainbow trout, Oncorhynchus mykiss(Walbaum), gills / J Fish Dis 17:601~611
Can A / 2007 / Concise review : human umbilical cord stroma with regard to the source of fetus-derived stem cells / Stem Cells 25:2886~2895
Freshney RI. / 2010 / Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications / Wiley-Blackwell
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Effect of dietary inclusion of the various sources of additives on growth, body composition and challenge test of juvenile rockfish (Sebastes schlegeli)
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Effect of two different dietary lipid sources on fatty acid composition and accumulation of rockfish (Sebastes schlegeli)
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DEVELOPMENT OF PCR ASSAY FOR DETECTION OF NEMATODA IN ROCKFISH Sebastes schlegeli
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Diet composition of Black rockfish, Sebastes schlegeli recaptured in the released area
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Low extrusion pressure and small feed particle size improve the growth performance and digestive physiology of Korean rockfish Sebastes schlegeli
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Apparent nutrient digestibility of mealworm meal for rockfish, Sebastes schlegeli
한국수산과학회 양식분과 학술대회
2016 .05
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Molecular characterization and expression profile of caspase-3 gene from Black rockfish Sebastes schlegeli upon the immune stimuli
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2015 .10
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Protein requirement in the microdiets for Korean rockfish (Sebastes schlegeli) larvae
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2016 .10
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Molecular characterization, phylogeny and expression of a CLEC4E in rockfish Sebastes schlegeli
한국수산과학회 양식분과 학술대회
2015 .10
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고수온에서 조피볼락(Sebastes schlegeli)의 산소소비 및 혈액성상 변화
수산해양교육연구
2016 .06
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Metagenomic analysis of intestinal microbiota after feeding the probiotics or prebiotics in Rockfish, Sebastes schlegeli
한국수산과학회 양식분과 학술대회
2015 .10
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Establishment and long-term culture of the cell lines derived from gonad tissues of Siberian sturgeon (Acipenser baerii)
Fisheries and Aquatic Sciences
2016 .01
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Lipid requirement in the granulated microdiets for Korean rockfish(Sebaste schlegeli) larvae
한국수산과학회 양식분과 학술대회
2016 .10
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