개인구독
소속 기관이 없으신 경우, 개인 정기구독을 하시면 저렴하게
논문을 무제한 열람 이용할 수 있어요.
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 202 - 207 (6page)
이용수
초록· 키워드
오류제보하기
The ideal cancer therapeutic strategy is to inhibit the tumor with minimal influence on the normal tissue. Recently, applying an alternating electric field for inhibiting tumor was developed; but, it has not been adopted to be one of the regular therapeutic options. More basic scientific evidence is needed to clarify the efficacy and safety. In the current study, co-culturing cancer cells and normal cells under the electrical stimulation was conducted to provide evidence of this novel cancer therapy. A microfluidic cell culture biochip has been developed and consisted of nine culture chambers incorporating with stimulating electrodes. Cells cultured in the chamber received uniform electric field and cell viability was studied during the culture course. The electric field perturbs cell division and the correlation between cell proliferation rate and inhibition effect was studied among five cell lines, i.e., Huh7, HeLa, TW06, BM1, and HEL299. The results confirmed that cells with higher proliferation rate responded to a higher inhibition. In addition, co-culturing cancer cells and normal cells was conducted to mimic in vivo microenvironment that consists of both cancer and stromal cells. The cancer cells and normal cells were respectively transduced with green fluorescent protein and red fluorescent protein in order to differentiate the cells in a same culture chamber. During the culture course, the electric field was applied to the culture chamber and both cells simultaneously received the field. The results indicated that the growth of the cancer cells were inhibited while the normal cells were maintained. These results provided the evidence of the therapeutic efficacy and safety. Moreover, the microfluidic cell culture biochip could be used for the systematic and precise investigations of the cellular responses under the electrical stimulation.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Kirson, E.D. / 2004 / Disruption of Cancer Cell Replication by Alternating Electric Fields / Cancer Res. 64:3288~3295
Kirson, E.D. / 2007 / Alternating electric fields arrest cell proliferation in animal tumor models and human brain tumors / Proc. Natl. Acad. Sci. USA 104:10152~10157
Karanam, N.K. / 2017 / Tumor-treating fields elicit a conditional vulnerability to ionizing radiation via the downregulation of BRCA1 signaling and reduced DNA double-strand break repair capacity in non-small cell lung cancer cell lines / Cell Death Dis. 8:e2711
Gera, N. / 2015 / Tumor treating fields perturb the localization of septins and cause aberrant mitotic exit / PLoS ONE 10:e125269
Giladi, M. / 2015 / Mitotic spindle disruption by alternating electric fields leads to improper chromosome segregation and mitotic catastrophe in cancer cells / Sci. Rep. 5:18046
Kirson, E.D. / 2007 / Alternating electric fields arrest cell proliferation in animal tumor models and human brain tumors / Proc. Natl. Acad. Sci. USA 104:10152~10157
Karanam, N.K. / 2017 / Tumor-treating fields elicit a conditional vulnerability to ionizing radiation via the downregulation of BRCA1 signaling and reduced DNA double-strand break repair capacity in non-small cell lung cancer cell lines / Cell Death Dis. 8:e2711
Gera, N. / 2015 / Tumor treating fields perturb the localization of septins and cause aberrant mitotic exit / PLoS ONE 10:e125269
Giladi, M. / 2015 / Mitotic spindle disruption by alternating electric fields leads to improper chromosome segregation and mitotic catastrophe in cancer cells / Sci. Rep. 5:18046
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Study on Cell Culture Process by using Single-use Cell Culture System
한국생물공학회 학술대회
2017 .04
-
Three-Dimensional Cell Culture Using Modified Natural Polymer for Cell Therapy
한국생물공학회 학술대회
2020 .06
-
Development of culture strategies for stem cell maintenance and differentiation through regulation of cell behaviors
한국생물공학회 학술대회
2016 .04
-
Development of Novel Suspending Cell Culture Substrates for ex vivo Cell-gene Therapy
한국생물공학회 학술대회
2018 .04
-
High-throughput Cell Culture Platform
한국생물공학회 학술대회
2021 .10
-
Deep Learning-Based Automated Analysis of NK Cell Cytotoxicity in Single Cancer Cell Arrays
BioChip Journal
2024 .09
-
A Novel Dual Culture Method for Effective Proliferation of Immune Cells Including Natural Killer Cells
한국생물공학회 학술대회
2022 .09
-
Fabrication of Cell Spheroids for 3D Cell Culture and Biomedical Applications
BioChip Journal
2023 .03
-
Human Cell Atlas and cell-type authentication for regenerative medicine
Experimental and Molecular Medicine
2020 .09
-
Enhanced Productivities in Animal Cell Technology : My Experiences
한국생물공학회 학술대회
2023 .10
-
Fluidized Bed Culture System Using Microcarriers for Adherent Cells
한국생물공학회 학술대회
2021 .10
-
Optimization of Cell Culture Process for Insect Cell
한국생물공학회 학술대회
2018 .10
-
FGF2-Mediated Insulin-Producing Beta Cells Differentiated from Human Omentum-Derived Stem Cells
한국생물공학회 학술대회
2022 .04
-
Development of a Column-Type Packed Bed Culture System for Culture of Adherent Cells
한국생물공학회 학술대회
2024 .09
-
Base technologies for Cell Engineering and Organ Chip
한국생물공학회 학술대회
2020 .10
-
Survivin protects fused cancer cells from cell death
BMB Reports
2017 .07
-
Development of Next-generation Cell-based Therapeutics for Diabetes
한국생물공학회 학술대회
2023 .04
-
The Use of Normal Stem Cells and Cancer Stem Cells for Potential Anti-Cancer Therapeutic Strategy
조직공학과 재생의학
2018 .01
-
Hyaluronic acid-based 3D cell culture system for stem cell proliferation
한국고분자학회 학술대회 연구논문 초록집
2015 .10
-
Understanding of molecular mechanisms in natural killer cell therapy
Experimental and Molecular Medicine
2015 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0