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논문 기본 정보

자료유형
학술저널
저자정보
Eliazar Alumbro Peniton Jr. (Department of Chemistry & Life Science BioScience Research Institute Sahmyook University) 엄유리 (Forest Medicinal Resources Research Center National Institute of Forest Science) 김현희 (Department of Chemistry & Life Science BioScience Research Institute Sahmyook University)
저널정보
한국육종학회 Plant Breeding and Biotechnology Plant Breeding and Biotechnology Vol.8 No.4
발행연도
2020.1
수록면
389 - 395 (7page)

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Genome sequencing has been going on major medicinal plants, including Platycodon grandiflorus, which is commonlyused as a functional food and medicinal resource in Korea. Molecular cytogenetic studies using fluorescence in situ hybridization(FISH) show the chromosomal organization of specific DNA sequences. We analyzed the FISH karyotypes of P. grandiflorus‘Jangbaek’ and a colchicine-induced tetraploid cultivar ‘Etteumbaek’ using 5S and 45S rDNA probes. As a result, ‘Jangbaek’ hadchromosome length ranging from 2.34 ± 0.13 to 3.99 ± 0.197 μm with its karyotypic formula of 2n = 2x = 18 = 16m + 2sm (twosatellites) whereas ‘Etteumbaek’ had chromosome length of 2.37 ± 0.08 to 4.20 ± 0.16 μm and a karyotypic formula of 2n = 4x = 36 =24m + 12sm (four satellites). A pair of 5S rDNA signals observed interstitially in chromosome 3 and the co-localization of 5S and 45SrDNA signals occupying the nucleolar-organizing region (NOR) of chromosome 6 was observed in the diploid cultivar ‘Jangbaek’. Doubling of both signals co-localized in the NOR of chromosome 6 were observed in the colchicine induced tetraploid cultivar. However, the tetraploid revealed a modified positioning of 5S rDNA signals on chromosome 3 interstitially, with observable vaguedispersed signals in one chromosome of chromosomes 1 and 5. This result will be useful for further breeding program and chromosomalbackbone for the Platycodon genome analysis.

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