지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.7
- 수록면
- 326 - 329 (4page)
이용수
초록· 키워드
오류제보하기
Background: Cultivated ginseng is often introduced as a substitute and adulterant of Russian wild ginseng due to its lower cost or misidentification caused by similarity in appearance with wild ginseng. The aim of this study is to develop a simple and reliable method to differentiate Russian wild ginseng from cultivated ginseng.
Methods: The mitochondrial NADH dehydrogenase subunit 7 (nad7) intron 3 regions of Russian wild ginseng and Chinese cultivated ginseng were analyzed. Based on the multiple sequence alignment result, a specific primer for Russian wild ginseng was designed by introducing additional mismatch and allelespecific polymerase chain reaction (PCR) was performed for identification of wild ginseng. Real-time allele-specific PCR with endpoint analysis was used for validation of the developed Russian wild ginseng single nucleotide polymorphism (SNP) marker.
Results: An SNP site specific to Russian wild ginseng was exploited by multiple alignments of mitochondrial nad7 intron 3 regions of different ginseng samples. With the SNP-based specific primer, Russian wild ginseng was successfully discriminated from Chinese and Korean cultivated ginseng samples by allele-specific PCR. The reliability and specificity of the SNP marker was validated by checking 20 individuals of Russian wild ginseng samples with real-time allele-specific PCR assay.
Conclusion: An effective DNA method for molecular discrimination of Russian wild ginseng from Chinese and Korean cultivated ginseng was developed. The established real-time allele-specific PCR was simple and reliable, and the present method should be a crucial complement of chemical analysis for authentication of Russian wild ginseng.
Methods: The mitochondrial NADH dehydrogenase subunit 7 (nad7) intron 3 regions of Russian wild ginseng and Chinese cultivated ginseng were analyzed. Based on the multiple sequence alignment result, a specific primer for Russian wild ginseng was designed by introducing additional mismatch and allelespecific polymerase chain reaction (PCR) was performed for identification of wild ginseng. Real-time allele-specific PCR with endpoint analysis was used for validation of the developed Russian wild ginseng single nucleotide polymorphism (SNP) marker.
Results: An SNP site specific to Russian wild ginseng was exploited by multiple alignments of mitochondrial nad7 intron 3 regions of different ginseng samples. With the SNP-based specific primer, Russian wild ginseng was successfully discriminated from Chinese and Korean cultivated ginseng samples by allele-specific PCR. The reliability and specificity of the SNP marker was validated by checking 20 individuals of Russian wild ginseng samples with real-time allele-specific PCR assay.
Conclusion: An effective DNA method for molecular discrimination of Russian wild ginseng from Chinese and Korean cultivated ginseng was developed. The established real-time allele-specific PCR was simple and reliable, and the present method should be a crucial complement of chemical analysis for authentication of Russian wild ginseng.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
References
참고문헌 (22)
참고문헌 신청
[학술지(정기간행물)] - Kennedy DO / 2003 / Ginseng : potential for the enhancement of cognitive performance and mood / Pharmacol Biochem Behav 75 : 687 ~ 700
[학술지(정기간행물)] - Rivera E / 2005 / The Rb1 fraction of ginseng elicits a balanced Th1 and Th2 immune response / Vaccine 23 : 5411 ~ 5419
[학술지(정기간행물)] - Lai DM / 2006 / Mediation of beta-endorphin by ginsenoside Rh2 to lower plasma glucose in streptozotocin-induced diabetic rats / Planta Med 72 : 9 ~ 13
[학술지(정기간행물)] - de Andrade E / 2007 / Study of the efficacy of Korean Red Ginseng in the treatment of erectile dysfunction / Asian J Androl 9 : 241 ~ 244
[학술지(정기간행물)] - Keum YS / 2000 / Antioxidant and anti-tumor promoting activities of the methanol extract of heatprocessed ginseng / Cancer Lett 150 : 41 ~ 48
[학술지(정기간행물)] - Rivera E / 2005 / The Rb1 fraction of ginseng elicits a balanced Th1 and Th2 immune response / Vaccine 23 : 5411 ~ 5419
[학술지(정기간행물)] - Lai DM / 2006 / Mediation of beta-endorphin by ginsenoside Rh2 to lower plasma glucose in streptozotocin-induced diabetic rats / Planta Med 72 : 9 ~ 13
[학술지(정기간행물)] - de Andrade E / 2007 / Study of the efficacy of Korean Red Ginseng in the treatment of erectile dysfunction / Asian J Androl 9 : 241 ~ 244
[학술지(정기간행물)] - Keum YS / 2000 / Antioxidant and anti-tumor promoting activities of the methanol extract of heatprocessed ginseng / Cancer Lett 150 : 41 ~ 48
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UCI(KEPA) : I410-ECN-0101-2018-524-001599024