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North American ginseng (Panax quinquefolius L.) was analysed for total ginsenosides and ten major ginsenosides (R0, Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, pseudoginsenoside F11, and gypenoside XVII), and variations in ginsenoside content with age of plant (over a four-year-period) and geographic location (Ontario versus British Columbia) were investigated. In the roots the total ginsenoside content increased with age up to 58-100 mgㆍg.1 dry weights in the fourth year, but in leaves it remained constant over time. Roots and leaves, moreover, had different proportions of individual ginsenosides. The most abundant ginsenosides were Rb1 (56 mgㆍg.1 for Ontario; 37 mgㆍg.1 for British Columbia) and Re (21 mgㆍg.1 for Ontario; 15 mgㆍg.1 for British Columbia) in roots, and Rd (28-38 mgㆍg.1), Re (20-25 mgㆍg.1), and Rb2 (13-19 mgㆍg.1) in leaves. Measurable quantities of Rf were found in leaves (0.4-1.8 mgㆍg.1) but not in roots or stems. Our results show that ginsenoside profiles in general, and Rf in particular, could be used for chemical fingerprinting to distinguish the different parts of the ginseng plant, and that ginseng leaves could be valuable sources of the ginsenosides Rd, Re, and Rb2.

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Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
ACKNOWLEDGEMENT
REFERENCES

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UCI(KEPA) : I410-ECN-0101-2009-524-013720378